Book Abstract
Fluorescence Methods for Investigation of Living Cells and Microorganisms, presents a comprehensive exploration of fluorescence-based techniques that allow non-destructive, in vivo analysis of cellular and microbial systems.
Combining theoretical foundations, cutting-edge techniques, and robust protocols, Fluorescence Methods for Investigation of Living Cells and Microorganisms is a vital reference for researchers, clinicians, and students. It equips users to employ fluorescence approaches effectively in live-cell analysis and microbial research.
The book outlines core principles of intrinsic and extrinsic fluorescence, guiding readers through the theoretical basis for capturing dynamic physiological and biochemical processes in living cells. It discusses the spectroscopic characteristics of fluorophores, photophysical phenomena, and the advantages of fluorescence in live-cell imaging .
Techniques such as fluorescence spectroscopy and fluorescence correlation spectroscopy (FCS) are evaluated for their utility in diagnosing microbial infections and probing molecular interactions at low concentrations, offering insights into kinetics and complexes in live systems.
The text provides detailed protocols for using vital fluorescent markers?including viability stains like propidium iodide and SYTOX?to distinguish live/dead cells. It covers their use in diverse systems such as microscopy and flow cytometry.
